What is inoculation process?

inoculation, process of producing immunity and method of vaccination that consists of introduction of the infectious agent onto an abraded or absorptive skin surface instead of inserting the substance in the tissues by means of a hollow needle, as in injection.

What is the process of inoculation in microbiology?

Inoculation may be defined as the process of adding effective bacteria to the host plant seed before planting. The purpose of inoculation is to make sure that there is enough of the correct type of bacteria present in the soil so that a successful legume-bacterial symbiosis is established.

What is an inoculation and what is its purpose?

In Immunology, inoculation is defined as the process of introducing an antigenic substance or vaccine into the body to trigger an immune response against a specific disease. Currently, the process of inoculation is also known as vaccination or immunization (Figure 1).

What is an example of inoculation?

For example, an inoculation message designed to discourage teen cigarette smoking (e.g., Pfau et al., 1992) might begin with a warning that peer pressure will strongly challenge their negative attitudes toward smoking, then follow this forewarning with a handful of potential counterarguments they might face from their …

How do you do an inoculation?

Youtube quote:The culture tube cap and drop the toothpick into the culture tube labeled with your plasma name and replace the cap make sure the toothpick does not touch the outside of the tube.

What is casting inoculation?

“Inoculation of molten cast iron” refers to the introduction of nuclei into the melt in order to influence the solidification process or structural formation in the casting in a specific way. Nuclei are fine particles that are ≤ 4 µm in size and which serve as crystallization centers for the graphite precipitation.

What is inoculation and incubation?

Inoculation is the process of introducing microorganisms or suspension of microorganisms into a culture medium. Incubation is the process of allowing inoculated microorganisms to grow under required growing conditions.

Why is it called inoculation?

Etymology. The term “inoculation” entered medical English through horticultural usage meaning to graft a bud (or eye) from one plant into another. It is derived from the Latin in + oculus (eye).

What is the difference between immunization and inoculation?

Immunization is the process of developing immunity. The word usually refers to immunity through vaccination, but it can occur through infection, as well. Inoculation is a synonym for vaccination and immunization. Vaccines teach your immune system to recognize and fight specific germs.

What is the difference between innoculation and vaccination?

Of the three words, vaccinate is the most narrow because it specifically means to give a vaccine to someone. Inoculate is more general and can mean to implant a virus, as is done in vaccines, or even to implant a toxic or harmful microorganism into something as part of scientific research.

What are inoculating tools?

An inoculation loop, also known as a smear loop, inoculation rod or microstreaker, is a simple tool used primarily by microbiologists to take and transfer a small sample (inoculum) of a microorganism culture, for example for stripping on a culture plate.

What is plate inoculation?

The principle of this method is the gradual dilution of an inoculum of bacteria over the surface of an agar plate to produce single isolated pure colonies. A small amount of sample is placed onto a section of the surface of an agar plate with either a sterile swab or flamed inoculating loop.

What are the different types of inoculation techniques?

Types of Inoculation

  • Direct Inoculation.
  • Covert Inoculation.
  • Indirect Inoculation.
  • Formal Inoculation.
  • Informal Inoculation.
  • Subliminal Inoculation.


What are inoculation tools in microbiology?

An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable.

What is inoculum in microbiology?

An inoculum can be defined as the population of microorganisms or cells that is introduced in the fermentation medium or any other suitable medium. From: Comprehensive Biotechnology (Second Edition), 2011.

What is incineration of inoculation loop?

The inoculating loop is sterilised by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat. In this way all contaminants on the wire are incinerated. Never lay the loop down once it is sterilised, or it may again become contaminated.

Why nichrome is used in inoculation loop?

Microbiologists use inoculating loops to transfer microorganisms to growth media. It is easy to sterilize and reuse because nichrome wire resists deterioration with repeated heat/cooling cycles.

What are inoculating loops made of?

A tool usually made of platinum or nichrome wire in which the tip forms a small loop with a diameter of about 5 mm, and is used to smear, streak, or take an inoculum from, a culture of microorganisms.

What are 5 aseptic techniques?

Procedures that involve aseptic technique include:

  • ‌Inserting PICC lines.
  • ‌Performing dialysis.
  • ‌Inserting catheters.
  • ‌Running IVs.
  • ‌Inserting chest tubes.
  • ‌Performing surgeries.
  • ‌Dressing wounds.


What is the difference between sterile and aseptic?

In the context of medicine, aseptic and sterile both mean germ-free. Aseptic is most commonly applied in the context of techniques and procedures, while sterile is most commonly used to describe environments and instruments that have been cleaned (sterilized). Sterile also means “incapable of producing offspring.”

What is difference between asepsis and aseptic?

People working in a variety of healthcare settings use aseptic technique. Aseptic technique is not the same as surgical asepsis, also known as sterile technique, which refers to a set of infection control practices that it is necessary to use in operating rooms.