What chemical constituent is responsible for the acid fast property of Mycobacterium species?

The acid-fastness of Mycobacteria is due to the high mycolic acid content of their cell walls, which is responsible for the staining pattern of poor absorption followed by high retention.

Which reagent is responsible for the acid-fast color of Mycobacterium species?

Acidfast Stain: Background and Introduction. Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol. Nearly all other genera of bacteria are nonacid-fast.

What substance is responsible for the acid fastness of an organism?

Mycolic acid is the mechanism responsible for the acid-fast property. The walls of the bacteria produce this waxy substance called mycolic acid. This acid links with substances within the cell walls of the bacteria, making them resistant to decolorization with acid alcohol treatment.

Why is the acid-fast stain used for Mycobacterium species?

Mycobacteria are called acid-fast because they resist decolorization with acid alcohol due to the nature of their complex cell wall. The acid-fast bacterial cell envelope is a modified version of the Gram-positive cell envelope with high lipid content.

What property of the acid-fast cell allows it to retain the primary stain?

Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat.

Is Bacillus subtilis acid-fast?

subtilis was acid-fast negative, whereas M. smegmatis was acid-fast positive. The acid-fast stain is classified under differential staining procedures that are used to pick out acid-fast organisms, including bacteria of the genus Mycobacterium.

What are the reagents used in the acid-fast stain?

The acid-fast bacilli will stain bright red, and the background will stain blue. Reagents used in the procedure include Ziehl–Neelsen carbol-fuchsin solution, 1% acid alcohol, and methylene blue solution [15].

Is Mycobacterium avium complex and acid fast bacilli?

In patients who may have pulmonary infection with Mycobacterium avium complex (MAC), diagnostic testing includes acid-fast bacillus (AFB) staining and culture of sputum specimens. If disseminated MAC (DMAC) infection is suspected, culture specimens should also include blood and urine.

Is Corynebacterium acid-fast?

Corynebacterium spp., members of the family Corynebacteriaceae, are Gram-positive, non-spore- forming, non-motile, aerobic, pleomorphic rods with coccoid or club-shaped appearance that are catalase-positive and non-acid-fast (Boone et al., 2001; Greenwood, 2007).

How do you get acid fast bacilli?

TB is spread from person to person through coughing or sneezing. TB can be latent or active. If you have latent TB, you’ll have TB bacteria in your body but won’t feel sick and can’t spread the disease to others. If you have active TB, you’ll have symptoms of the disease and could spread the infection to others.

What is the principle behind acid-fast staining?

Principle of acid fast staining (in mycobacteria):

Heat softens the wax in the cell wall and allows the stain (basic fuchsin) to enter. The fuchsin dye is more soluble in phenol than in water or alcohol. Phenol in turn is more soluble in lipids or waxes, thus the dye-phenol mixture enters the cell.

What is the purpose of acid-fast staining quizlet?

What is the purpose of an Acid-Fast stain? It is a differential stain used to detect cells capable of retaining a primary stain when treated with an acid alcohol.

Which methods are used in acid-fast staining?

Acid-fast structures can be visualized under a microscope using two principal methods, the carbolfuchsin staining, and the fluorochrome procedure. The carbolfuchsin staining comprises of the Ziehl-Neelsen method and the Kinyoun method.

How might the acid-fast characteristic of Mycobacterium?

How might the acid-fast characteristic of Mycobacterium enhance the organism’s ability to cause disease? because they have a waxy coating around their cell that prevents alot of different things from getting into the cell. Including antibiotics if a person is sick.

What are the three components of an acid-fast stain?

Acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium. Acid-fast organisms are characterized by wax-like, nearly impermeable cell walls; they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.

Who developed acid-fast staining?

Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.

What is chemically unique about the Mycobacterium genus that causes it to be acid-fast?

The acid-fastness of Mycobacteria is due to the high mycolic acid content of their cell walls, which is responsible for the staining pattern of poor absorption followed by high retention. Some bacteria may also be partially acid-fast, such as Nocardia.

Which specific Mycobacterium organism was Ehrlich trying to stain when he developed the acid-fast stain?

mycobacterium tuberculosis organism

When Robert Koch announced the discovery of a staining method to demonstrate the mycobacterium tuberculosis organism in 1882, Ehrlich soon improved upon the method by staining the organism with aniline basic fuchsine and decolorizing it in a mineral acid, hence the “acid fast” tag.