Is Haemophilus influenzae anaerobic?

H. influenzae, a fastidious, pleomorphic, gram-negative coccobacillus, requires hemin (X factor) and nicotinamide- adenine-dinucleotide (NAD, also known as V factor) for in vitro growth. It is generally aerobic, but can grow as an anaerobe under certain conditions.

Is Haemophilus influenzae an anaerobe?

H. influenzae grows aerobically and as a facultative anaerobe. During aerobic growth, the organism experiences oxidative stress derived from its own metabolism. As both a commensal and a pathogen of the human upper respiratory tract, H.

Is Haemophilus aerobic or anaerobic?

Haemophilus spp. are small, pleomorphic, nonmotile, nonsporing gram-negative rods or GNCB. They are aerobic and facultatively anaerobic, and addition of 5–10% carbon dioxide to the incubation atmosphere may enhance growth. The oxidase and catalase reactions vary.

Does Haemophilus grow anaerobically?

All Haemophilus are gram-negative, aerobic or facultative anaerobic and nonmotile and require a growth factor that is found in blood. They are minute in size, H. influenzae measuring 0.3 micrometre across and up to 2 micrometres long.

What are the characteristics of Haemophilus influenzae?

H. influenzae appear as large, round, smooth, convex, colorless-to-grey, opaque colonies on a CAP (Figure 1). Encapsulated strains appear more mucoidal than non-encapsulated strains, which appear as smaller, compact grey colonies. No hemolysis or discoloration of the CAP is apparent.

Is H. influenzae capsulated?

Haemophilus influenzae is a pleomorphic gram-negative coccobacillus. H. influenzae may be either encapsulated (typeable) or unencapsulated (nontypeable). There are 6 encapsulated serotypes (designated a through f) that have distinct capsular polysaccharides.

What kind of bacteria is Haemophilus influenzae?

Haemophilus influenzae is a bacteria characterized as a small, facultatively anaerobic, pleomorphic, and capnophilic gram-negative coccobacillus of the family Pasteurellaceae. It is a common cause of a variety of invasive and non-invasive bacterial infections.

Does Haemophilus ferment glucose?

H. influenzae ferments glucose, maltose, and laevulose to acid without gas. Proteolytic activity is very slight, but many strains produce indole.

Is Mycoplasma pneumoniae aerobic or anaerobic?

In general, mycoplasmas are facultative anaerobes, except for Mycoplasma pneumoniae, which is a strict aerobe [21, 32]. The presence of the anaerobic form of these microorganisms is usually explained by the availability of oxygen and by the colonization of microhabitats protected from air.

Is Pseudomonas aeruginosa aerobic or anaerobic?

Pseudomonas aeruginosa had been considered as an obligately aerobic bacterium previously, but it is now recognized to be highly adapted to anaerobic conditions. Because the P. aeruginosa-infected mucus in the CF airway is depleted of oxygen, the anaerobic physiology of P.

Is Haemophilus influenzae a commensal?

Haemophilus influenzae is present as a commensal organism in the nasopharynx of most healthy adults from where it can spread to cause both systemic and respiratory tract infection.

Does Haemophilus influenzae grow on MacConkey?

Haemophilus spp.
do not grow on MacConkey agar. Haemophilus influenzae has been shown to cause invasive infection such as meningitis and there is direct spread from water droplets from the upper respiratory tract of infected individuals. The development of the H.

Is Haemophilus influenzae indole positive?

Thus, indole-positive, nonhemolytic Haemophilus isolates in respiratory cultures can be presumptively identified as H. influenzae.

Is Haemophilus a lactose fermenter?

Facultatively anaerobic; fermentative and respiratory type metabolism. Glucose, not lactose, is fermented.

Is Haemophilus influenzae lactose fermenting?

aureus release V-Factor during growth, which defuses into the surrounding medium, enhancing the growth of H. influenzae. influenzae will show Satellitism.

Test Reaction
Galactose +

Is Haemophilus influenzae urease positive?

It is evident that Haemophili form a potent urease during growth in vitro, and that this urease is capable of giving a positive urease test without further growth of the organisms.
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What are the biochemical test for Haemophilus influenzae?

Biochemical Test of Haemophilus influenzae

Basic Characteristics Properties (Haemophilus influenzae)
Catalase Positive (+ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gram Staining Gram-negative

Why does Haemophilus influenzae not grow on blood agar?

Haemophilus influenzae requires hemin (factor X) and NAD+ (factor V) for growth. Other Haemophilus species require only NAD+ and therefore grow on blood agar.